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Pranam Chatterjee

@pranamanam

Jul 13

5 tweets
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After almost three years of painstaking effort, today, I'm excited to present my team's amazing work on specifying human oogonia via combinatorial transcription factor induction in iPSCs! πŸ₯³ An incredible collaboration with my good friend @Christian Kramme, PhD! biorxiv.org/content/10.110…

Germ cells are the vehicle of human reproduction, arising early in embryonic development and developing throughout adult life until menopause onset in women. Primordial germ cells are the common...

biorxiv.org/content/10.110…

Efficient Human Germ Cell Specification from Stem Cells via Combinatorial Expression of Transcrip...

Germ cells are the vehicle of human reproduction, arising early in embryonic development and developing throughout adult life until menopause onset in women. Primordial germ cells are the common...

It all started with a graph theory algorithm to identify "central" TFs in the oogenesis gene regulatory network, and later turned into an all-out screening effort in primordial germ cells (hPGCLCs), leading to publications in @Cell Reports Methods and @StemCellReports. πŸ’»β†’πŸ§«

In the end, we identified a subset of TFs (termed DNR3) that, when overexpressed in hiPSCs, generate induced oogonia/oocyte-like cells (iOLCs)! Our iOLCs can be maintained in feeder-free conditions and be expanded post-isolation. πŸ’ͺ🏾

During all of this, I co-founded @Gameto alongside @Martin Varsavsky πŸ‡ΊπŸ‡¦, and we raised ~$2 million for this effort, which is now being spearheaded by @Christian Kramme, PhD and @Dina Radenkovic, who recently raised an amazing Series A round for the company! 🀩 gametogen.com

Alongside my incredible co-authors/students, we are now underway with the arduous review process! πŸ˜… Please take a read and let us know of your thoughts. We have a long way to go, but would love to have you join us on the path toward female reproductive longevity. 🌟

Pranam Chatterjee

@pranamanam

Designing proteins to do powerful things! Computational #CRISPR/Protein/Cell Engineer πŸ§¬πŸ’»πŸ§« Assistant Professor at @DukeU | Formerly @medialab @harvardmed

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